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This work proposes an algorithm aimed at recognizing and accounting Koch bacilli in digital images of microbiological sputum samples stained with auramine, in order to determine the degree of concentration and the state of the disease (tuberculosis). The algorithm was developed with the main objective of maximizing the sensitivity and specificity of the analysis of microbiological samples (recognition and counting of bacilli) according to each preparation method (direct and diluted pellets) in order to reduce the subjectivity of the visual inspection applied by the specialist at the time of analyzing the samples. The proposed algorithm consists of a background removal, an image improvement stage based on consecutive morphological closing operations, a segmentation stage of objects of interest based on thresholdization and a classification stage based on SVM. Each algorithmic stage was developed taking into account the method of preparation of the sample to be processed, being this aspect the main contribution of the proposed work, since it was possible to achieve very satisfactory results in terms of specificity and sensitivity. In this context, sensitivity levels of 91.24% and 93.79% were obtained. Specificity levels of 90.33% and 94.85% were also achieved for direct and diluted pellet methods respectively.

The objective of this study is to understand and analyze the consequences of an ethical crisis caused by the unethical behavior of employees at a banking company, which demonstrates a lack of commitment and poor alignment with the organization's values. The research examines the perceptions of employees, clients and non-clients regarding the media crisis and the communication response provided by the company to its stakeholders. Finally, the research offers recommendations on how to effectively manage a media crisis. Design/methodology/approach The study analyzed, through 1,182 questionnaires, the perceptions of employees, clients and non-clients regarding the ethical crisis caused by the employees of a bank in Peru regarding the pre-sale of tickets for the Peru vs. Colombia match in the Russia 2018 qualifiers. The study was complemented by in-depth interviews with two key executives of the company. Findings The results showed that stakeholders react differently to the company's response to the crisis caused by employee misconduct. The study reflected that those who felt most affected by the incidents and perceived the crisis to be more serious were employees, as opposed to clients and non-clients. In addition, the results revealed significant differences in stakeholders' perceptions of how the company handled the crisis, with non-clients being the most critical. Research limitations/implications Although the case concerned a single company in Latin America, due to the media nature of the facts, there was diverse and important information that allowed us to analyze the internal part of the company and evaluate the impact of the crisis on the company's image and reputation outside the company, specifically with customers and non-customers. Likewise, the study proposed recommendations and communication strategies to respond to a crisis. Practical implications The research offered a series of recommendations consisting of a set of strategies for companies and their leaders to deal with business crises caused by negative employee behavior. Social implications The study analyzed how an ethical crisis situation can lead to a media crisis, which can damage the brand and the company's reputation. It can also have a social impact, since the marketing strategy with good intentions on the part of the banking company became a negative action at a social level, generating disorder, disbelief and discomfort in a matter of national interest, such as the match between the Peruvian and Colombian national football teams in qualifying for the 2018 World Cup in Russia. Originality/value The research analyzed the relationship between corporate culture and the creation of commitment in employees as a key tool to prevent possible business crises due to lack of trust and integrity of employees; which can prevent a possible crisis in the image and reputation of the company.

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