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Item type:Publication, Comparison between clinical and computational method of surface measurements of skin ulcers caused by cutaneous leishmaniasis(Sociedad Espanola de Optica, 2022-01-01)Cutaneous leishmaniasis is a neglected disease caused by an intracellular parasite of the genus Leishmania. The implementation of new techniques for its monitoring is increasing. This article presents a methodology based on photogrammetry for the follow-up and evaluation of skin ulcers caused by cutaneous leishmaniasis. Leishmaniasis was experimentally inoculated into golden hamsters, which were analyzed during and after treatment for four months. The follow-up of the evolution of the disease is carried out by taking clinical (manual) and computational measurements. The clinical data were obtained using a caliper, measuring directly on the ulcerated area and performing calculations of geometric approximations with sizes similar to the ulcerated area (square and elliptical). Computational data was obtained by Agisoft PhotoScan software using a photogrammetry-based 3D reconstruction technique to model and extract information from the surface. Validation is carried out on the size of the cutaneous ulcerative areas under study, comparing the square and elliptical approximation of the area with that calculated by the software (total area of the 3D surface generated from the point cloud). This validation is performed by graphically comparing the trend in the variation of the ulcer area of each hamster, and an area correlation calculation (clinical vs computational measurements). As a result, 60% of the study population presents a strong correlation with a value of 0.8. We found that the computational technique allows physicians to provide a more reliable concept during ulcer healing follow-up - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Temporal Evaluation of the Surface Area of Treated Skin Ulcers Caused by Cutaneous Leishmaniasis and Relation with Optical Parameters in an Animal Model: A Proof of Concept(Multidisciplinary Digital Publishing Institute (MDPI), 2023-07-01)Cutaneous leishmaniasis (CL) is a neglected disease caused by an intracellular parasite of the Leishmania genus. CL lacks tools that allow its understanding and treatment follow-up. This article presents the use of metrical and optical tools for the analysis of the temporal evolution of treated skin ulcers caused by CL in an animal model. Leishmania braziliensis and L. panamensis were experimentally inoculated in golden hamsters, which were treated with experimental and commercial drugs. The temporal evolution was monitored by means of ulcers’ surface areas, as well as absorption and scattering optical parameters. Ulcers’ surface areas were obtained via photogrammetry, which is a procedure that allowed for 3D modeling of the ulcer using specialized software. Optical parameters were obtained from a spectroscopy study, representing the cutaneous tissue’s biological components. A one-way ANOVA analysis was conducted to identify relationships between both the ulcers’ areas and optical parameters. As a result, ulcers’ surface areas were found to be related to the following optical parameters: epidermis thickness, collagen, keratinocytes, volume-fraction of blood, and oxygen saturation. This study is a proof of concept that shows that optical parameters could be associated with metrical ones, giving a more reliable concept during the assessment of a skin ulcer’s healing.