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Item type:Publication, Bioactive membranes of polymeric micro and nanocomposites prepared with the natural anionic marine polysaccharide (Alginate) functionalized with extracts of cat’s claw (Uncaria tomentosa) and Aloe vera(Trans Tech Publications Ltdttp@transtec.ch, 2020-01-01)In this research, the nanostructured alginate (AL) membranes were prepared with natural bioactive compound, Cat's claw (Uncaria tomentosa) extract (UT). UT is broadly used as an anti-inflammatory agent and the effect on the treatment of Rheumatism was proved by many scientists. For this reason, we added this bioactive compound in the process of AL membrane formulation to improve the biological activities. 2-dimentional (2-D) and 3-dimentional (3-D) AL membranes were prepared with and without addition of UT extracts. 3-D AL membranes were prepared using ultrasound with high intensity. The wettability of AL membranes depending on the concentration of AL was studied by measuring contact angle and surface energy. Stabilization agent, poloxamer 407, was used to improve the stability of AL nanoemulsion. The effects of UT in 3-D AL membranes were studied by measuring swelling behavior and contact angle. The surface morphology was measured with scanning electron microscopy (SEM). Comparing to 2-D AL membranes, 3-D AL membranes presented rougher surface due to AL nanoparticles presence. When UT was incorporated in AL membranes, strong antioxidant activity and higher contact angle and swelling ratio were observed than non-UT incorporated AL membranes. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Ultrasound-assisted anthocyanins extraction from purple cabbage for optical biosensor of wound healing monitoring(Trans Tech Publications, 2024-03-29)The ethanol-water based extraction of phenolic bioactive components, anthocyanins, from purple cabbage (PC) was carried out. Ultrasound assisted extraction was performed and each cycle was set to 3 minutes. UV-visible absorbance was evaluated at each cycle (total 3 cycles) and the intensity of absorbance at 546~550 nm was interpreted as the extraction efficiency of anthocyanin. The pH sensibility of PC extracts was evaluated at pH 4, 5, 6, 7, and 8 and measured with UV-Visible spectrometry. Each pH condition, the extracts presented different color shades and wavenumbers of maximum absorbance proving their pH sensibility. Antioxidant activity of PC extract was evaluated and the excellent inhibition capacity was observed (~98%). PC extract was then embedded in alginate membrane to use as wound dressing materials. Alginate membrane with PC extract also presented high pH sensibility showing remarkable color changes after exposure to different pH environments. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Three-Dimensional Membranes of Natural Polymer Complex Nanoparticle for Potential Medical Applications(Multidisciplinary Digital Publishing Institute (MDPI), 2023-11-01)Skin wound healing is a complex biological process of tissue regeneration in which the wound dressing is crucial for rapid healing; it must protect the wound keep an adequate level of moisture and prevent infections. Alginate (AL), a polysaccharide from brown algae, has been extensively studied for wound treatment, and aloe vera gels (AVGs) have also been used in the treatment of skin. The AVG main bioactive polysaccharide was combined with AL for the preparation of membranes. Two-dimensional membranes were prepared by casting and, for comparison, transparent nanoparticle 3D membranes were produced by high-intensity ultrasonication followed by ionotropic crosslinking. The effects of the amount of AVG, ionotropic gelation, and the structure (2D or 3D) of the AL-AVG membranes were compared. Scanning electron microscopy (SEM) showed higher surface roughness on 3D membranes. Three-dimensional membranes showed a higher swelling ratio, and swelling increased with AVG content and decreased with higher calcium concentration and longer gelation times. The degradation of the membranes was evaluated with and without a lysozyme at pH 5.5, 7.5, and 8.5, to simulate different skin conditions; the results evidence that pH had a higher effect than the enzyme. The cytotoxicity of the membranes was evaluated with ATCC CCL 163 and ATCC CCL 81 cells, and an excellent biocompatibility of both cell types (>90% of cell viability after 48 h incubation) was observed for all AL-AVG membranes. - Some of the metrics are blocked by yourconsent settings
Item type:Publication, Chitosan–Alginate Nanoparticle System for the Delivery of Cocoa Polyphenols for Diabetes and Obesity Management(Wiley, 2026-06-15)Phenolic-rich extracts from Theobroma cacao are obtained under different solvent extraction conditions, that is, with ethanol solo (first-stage extract [FSE]) and with ethanol-acetone in two steps (second-stage extract [SSE]) to maximize bioactive compound recovery. Among the evaluated solvents, acetone yields the highest total phenolic content (532.57 ?g GAE/g), and the corresponding extract exhibited strong inhibitory activity against ?-glucosidase (IC 50 = 4.04 ?g/mL) and lipase (IC 50 = 14.034 ?g/mL), highlighting its potential role in the prevention of metabolic diseases. However, phenolic compounds often present limited stability and bioavailability, which may restrict their practical application. To address this limitation, cocoa phenolic extracts were encapsulated within chitosan–alginate nanoparticles (CANPs) using a polyelectrolyte complexation approach. The obtained nanoparticles exhibited particle sizes ranging from 328.5 to 454.9 nm for FSE and SSE formulations, respectively, along with high absolute zeta potential values (> |32| mV), indicating good colloidal stability. The extraction method significantly influenced the encapsulation efficiency (EE), with SSE-CANPs showing substantially higher EE (64.45% ± 0.49%) compared to FSE-CANPs (33.93% ± 0.24%). The in vitro release of phenolic compounds was evaluated under simulated gastrointestinal conditions. The total cumulative release remained below 50%, indicating high resistance to rapid degradation and enabling a sustained release profile under varying pH conditions.